Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,632,437 (T)7→6 intergenic (‑71/+8) BW25113_RS08135 ← / ← BW25113_RS25620 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,632,4310T.100.0% 59.0 / NA 15intergenic (‑65/+14)BW25113_RS08135/BW25113_RS25620hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/8);  total (7/8)

GGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGA  >  NZ_CP009273/1632353‑1632502
                                                                              |                                                                       
ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCatgat                                                             <  1:343060/90‑1 (MQ=255)
ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCatgat                                                             <  2:402953/90‑1 (MQ=255)
        aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCgt                                                     <  1:65412/90‑1 (MQ=255)
                            cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                 >  1:160007/1‑90 (MQ=255)
                             aGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCgt                                                     <  1:271493/69‑1 (MQ=255)
                             aGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCgt                                                     >  2:271493/1‑69 (MQ=255)
                              gTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAAt                               >  1:115602/1‑90 (MQ=255)
                                cATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAg                             >  2:295424/1‑90 (MQ=255)
                                       ggCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCaa                      <  2:114788/90‑1 (MQ=255)
                                                   gctgGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGt                                                           <  1:34613/41‑1 (MQ=39)
                                                   gctgGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGt                                                           >  2:34613/1‑41 (MQ=39)
                                                   gctgGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCgtag                                                   >  1:258694/1‑49 (MQ=39)
                                                   gctgGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCgtag                                                   <  2:258694/49‑1 (MQ=39)
                                                     tgGTTCAGAGTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTccc        >  2:66235/1‑90 (MQ=255)
                                                           agagTTAAAAAAACTATGATTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGa  <  1:244637/90‑1 (MQ=255)
                                                                              |                                                                       
GGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGA  >  NZ_CP009273/1632353‑1632502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: