Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,760,505	(T)5→4	coding (2438/3057 nt)	ydiJ ←	FAD‑binding and (Fe‑S)‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,760,501	0	T	.	100.0%	28.3 / NA	8	coding (2442/3057 nt)	ydiJ	FAD‑binding and (Fe‑S)‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (2/6);  total (2/6)

CCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGC  >  NZ_CP009273/1760439‑1760583                                                               |                                                                                   ccTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGcca                                                        <  2:350508/90‑1 (MQ=255)           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTgcgc                                              <  1:312728/90‑1 (MQ=255)           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTgcgc                                              <  2:93868/90‑1 (MQ=255)                    tGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATa                                     >  1:112726/1‑90 (MQ=255)                                        aGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGca                 <  2:231086/90‑1 (MQ=255)                                                 ggCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGca                 >  1:394382/1‑81 (MQ=255)                                                 ggCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGca                 <  2:394382/81‑1 (MQ=255)                                                       aaaCCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTgc  <  2:49893/90‑1 (MQ=255)                                                               |                                                                                   CCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGC  >  NZ_CP009273/1760439‑1760583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑