Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,417,788	(C)6→7	coding (69/120 nt)	BW25113_RS26130 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,417,782	1	.	C	100.0%	23.9 / NA	10	G25G (GGA→GGG)	BW25113_RS26130	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (5/5);  total (5/5)

GTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA  >  NZ_CP009273/1417712‑1417862                                                                        |                                                                                 gTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGaca                                                                >  1:90513/1‑90 (MQ=255)     cacaTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGc                                                            <  1:274002/90‑1 (MQ=11)                 gTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGatta                                                >  1:19573/1‑90 (MQ=255)                 gTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGatta                                                >  2:32783/1‑90 (MQ=255)                       cagtcagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCAc                                          <  2:215964/90‑1 (MQ=255)                                       ccaccaCCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTg                          >  2:161443/1‑90 (MQ=255)                                       ccaccaCCGCATCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCAc                                          >  1:69871/1‑74 (MQ=11)                                       ccaccaCCGCATCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCAc                                          <  2:69871/74‑1 (MQ=11)                                                       cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc          <  1:227525/90‑1 (MQ=255)                                                               aTGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa  <  1:151552/90‑1 (MQ=255)                                                                        |                                                                                 GTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA  >  NZ_CP009273/1417712‑1417862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑