| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,736 | A→T | intergenic (+105/‑87) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,736 | 0 | A | T | 95.8% | 83.2 / ‑3.2 | 24 | intergenic (+105/‑87) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (10/13); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTC > NZ_CP009273/49656‑49821 | tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCa < 1:7905/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 1:161537/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAAAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 2:33361/90‑1 (MQ=255) cTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATcc > 1:19924/1‑90 (MQ=255) cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 1:210814/90‑1 (MQ=255) gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg < 1:393939/90‑1 (MQ=255) ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt < 2:201727/90‑1 (MQ=255) gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt > 1:88946/1‑90 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga > 1:308611/1‑76 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 2:308611/76‑1 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa > 2:267703/1‑90 (MQ=255) gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTACTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa < 2:397803/88‑1 (MQ=255) gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTACTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa > 1:397803/1‑88 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc < 2:132336/38‑1 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc > 1:132336/1‑38 (MQ=38) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca > 2:196651/1‑45 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca < 1:196651/45‑1 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 1:360348/59‑1 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga > 2:360348/1‑59 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt > 2:172194/1‑90 (MQ=255) gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa < 2:19924/90‑1 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa < 1:83781/90‑1 (MQ=255) aaTATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa < 2:7672/90‑1 (MQ=255) taAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc > 2:373787/1‑90 (MQ=255) | TACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTC > NZ_CP009273/49656‑49821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |