Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,122,528	T→G	T202P (ACC→CCC)	wcaE ←	colanic acid biosynthesis glycosyltransferase WcaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,122,528	0	T	G	100.0%	26.0 / NA	9	T202P (ACC→CCC)	wcaE	colanic acid biosynthesis glycosyltransferase WcaE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/4);  total (5/4)

AAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCG  >  NZ_CP009273/2122457‑2122614                                                                        |                                                                                       aaaGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGa                                                                      >  1:175213/1‑90 (MQ=255)                        gTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGa                                               <  1:37247/90‑1 (MQ=255)                                aCTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTa                                       <  2:177255/90‑1 (MQ=255)                                   tttttCGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAAt                                    >  1:92724/1‑90 (MQ=255)                                           gTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAAcc                            <  2:29057/90‑1 (MQ=255)                                                                attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc       >  1:335654/1‑90 (MQ=255)                                                                attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc       <  1:358773/90‑1 (MQ=255)                                                                attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc       >  2:158062/1‑90 (MQ=255)                                                                attGGTGGGAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGc       >  2:54590/1‑90 (MQ=255)                                                                     tgggAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAgcg  >  2:130947/1‑90 (MQ=255)                                                                        |                                                                                       AAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTTCGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCG  >  NZ_CP009273/2122457‑2122614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑