Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,196,055	T→C	V766V (GTT→GTC)	BW25113_RS11045 →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,196,055	0	T	C	100.0%	27.6 / NA	10	V766V (GTT→GTC)	BW25113_RS11045	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/4);  total (6/4)

CCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTTATAGCGGGCTGGAACAACTGAA  >  NZ_CP009273/2195992‑2196138                                                                |                                                                                    cccTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCgg                                                           >  1:236012/1‑90 (MQ=255)         tCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAAtgc                                                   <  2:170544/90‑1 (MQ=255)           tGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAAtgctg                                                 <  2:365733/90‑1 (MQ=255)                 ccGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCttt                                           >  2:198119/1‑90 (MQ=255)                            gTCCAGCCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAgggggg                                <  2:141564/90‑1 (MQ=255)                              ccAGGCCGCGCCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGAc                              <  2:29363/90‑1 (MQ=255)                                     gcgcCTTATCACTAATAGTCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTta                       >  2:80567/1‑90 (MQ=255)                                      cgcCTTATCACTAATAATCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTtat                      >  2:209117/1‑90 (MQ=255)                                                  aataatCAAACTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTTATAGCGGGCTGGaa          >  2:159602/1‑90 (MQ=255)                                                          aaCTGTCACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTTATAGCGGGCTGGAACAACTGaa  >  2:49340/1‑90 (MQ=255)                                                                |                                                                                    CCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGGACGTTTTTATAGCGGGCTGGAACAACTGAA  >  NZ_CP009273/2195992‑2196138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑