Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,220,044	A→G	S236P (TCG→CCG)	yohF ←	SDR family oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,220,044	0	A	G	100.0%	50.9 / NA	16	S236P (TCG→CCG)	yohF	SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/6);  total (10/6)

TGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGC  >  NZ_CP009273/2219963‑2220126                                                                                  |                                                                                   tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCgg                                                                            <  2:118230/90‑1 (MQ=255) tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCgg                                                                            <  2:283096/90‑1 (MQ=255)          cACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAAt                                                                   >  2:20894/1‑90 (MQ=255)                    aCTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTc                                                         <  1:155330/90‑1 (MQ=255)                        cgcTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                                     >  1:271218/1‑90 (MQ=255)                          cTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACa                                                   >  1:92034/1‑90 (MQ=255)                            aTTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaaa                                                 >  1:35282/1‑90 (MQ=255)                                    ttGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCACGGTGGGGTAATTTGCGCCCTCCGAACAAAGCCAcacc                                         >  2:187467/1‑90 (MQ=255)                                      gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGccc                                                                              <  1:129826/51‑1 (MQ=255)                                      gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGccc                                                                              >  2:129826/1‑51 (MQ=255)                                      gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                       >  2:105336/1‑90 (MQ=255)                                      gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGGGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                       >  2:260087/1‑90 (MQ=255)                                           gtgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCgg                                                                            <  1:336061/48‑1 (MQ=255)                                           gtgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCgg                                                                            >  2:336061/1‑48 (MQ=255)                                                            aaGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGtt                 >  1:205688/1‑90 (MQ=255)                                                                           aTCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAAcgccgc  <  1:20894/90‑1 (MQ=255)                                                                                  |                                                                                   TGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGC  >  NZ_CP009273/2219963‑2220126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑