Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,435,707	C→G	G155G (GGC→GGG)	mnmC →	bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,435,707	0	C	G	87.5%	18.8 / ‑2.3	8	G155G (GGC→GGG)	mnmC	bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (5/2);  total (5/3)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

AGGCGCAGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCG  >  NZ_CP009273/2435625‑2435791                                                                                   |                                                                                     aGGCGCAGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGGGATATTa                                                                               >  1:221444/1‑90 (MQ=255)       aGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAAc                                                                         <  2:343417/90‑1 (MQ=255)              aaTGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGGGATATTAACGAACTGACCAg                                                                  <  2:221444/90‑1 (MQ=255)                                              tCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGGGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAg                                  >  2:174137/1‑90 (MQ=255)                                                               aCGCTGGATTTATGGTTTGGGGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGAc                 <  1:53388/90‑1 (MQ=255)                                                                    ggATTTATGGTTTGGGGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCtt            >  1:152207/1‑90 (MQ=255)                                                                     gATTTATGGTTTGGGGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCttt           >  1:292103/1‑90 (MQ=255)                                                                              tttGGGGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCg  >  1:8392/1‑90 (MQ=255)                                                                                   |                                                                                     AGGCGCAGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCG  >  NZ_CP009273/2435625‑2435791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑