| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,725,700 | T→G | K611N (AAA→AAC) | clpB ← | ATP‑dependent chaperone ClpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,725,700 | 0 | T | G | 83.3% | 27.1 / 0.1 | 12 | K611N (AAA→AAC) | clpB | ATP‑dependent chaperone ClpB |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (4/6); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01 | |||||||||||
TCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAG > NZ_CP009273/2725617‑2725785 | tcCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTcccc < 1:214359/90‑1 (MQ=255)tcCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTcccc < 1:266714/90‑1 (MQ=255) ggACATATCGATACGGACCATAGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTgg > 2:269325/1‑90 (MQ=255) tatCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGa < 2:249623/90‑1 (MQ=255) aTCGCCTCGTCGCTATCCAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGaa > 2:248793/1‑90 (MQ=255) aTCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGaa > 2:19353/1‑90 (MQ=255) aTCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGAtt < 1:308713/90‑1 (MQ=255) tAAAGTTCGCCAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCg > 2:319444/1‑90 (MQ=255) cAGCGCCTTACAAAGCTCTGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGc < 1:133907/90‑1 (MQ=255) aCAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTacg > 1:285274/1‑90 (MQ=255) ctctGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAAt < 1:345711/90‑1 (MQ=255) ctGTGTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAg < 1:164253/90‑1 (MQ=255) | TCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAG > NZ_CP009273/2725617‑2725785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |