Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,849,816	T→C	E117G (GAA→GGA)	ygbA ←	nitrous oxide‑stimulated promoter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,849,816	0	T	C	100.0%	32.7 / NA	11	E117G (GAA→GGA)	ygbA	nitrous oxide‑stimulated promoter family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/6);  total (5/6)

GCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAC  >  NZ_CP009273/2849742‑2849889                                                                           |                                                                          gCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCg                                                            >  1:274184/1‑90 (MQ=255)                             aTTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCAc                                <  2:357603/90‑1 (MQ=255)                                 aaCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGa                            >  1:240215/1‑90 (MQ=255)                                  aCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGAc                           <  1:120180/90‑1 (MQ=255)                                            gCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATc                 >  1:280973/1‑90 (MQ=255)                                                 aCAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatga            <  1:378255/90‑1 (MQ=255)                                                         gCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                          >  1:256593/1‑52 (MQ=255)                                                         gCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                          <  2:256593/52‑1 (MQ=255)                                                           cTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAc  <  2:123283/90‑1 (MQ=255)                                                            tGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa   <  1:50003/88‑1 (MQ=255)                                                            tGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa   >  2:50003/1‑88 (MQ=255)                                                                           |                                                                          GCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAC  >  NZ_CP009273/2849742‑2849889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑