Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	354,025	T→C	E126G (GAA→GGA)	cynR ←	transcriptional regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	354,025	0	T	C	100.0%	50.5 / NA	16	E126G (GAA→GGA)	cynR	transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/10);  total (6/10)

GCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGC  >  NZ_CP009273/353939‑354094                                                                                       |                                                                      gCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCt                                                                    <  1:97729/90‑1 (MQ=255)               acaGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTg                                                      <  2:55119/90‑1 (MQ=255)                 aGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGAt                                                    <  2:379925/90‑1 (MQ=255)                  ggCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATg                                                   >  1:112566/1‑90 (MQ=255)                        aaGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGa                                             <  2:241001/90‑1 (MQ=255)                                         cAACTCGTCGGGGCAAAGCATATCCTTGATTTTCCCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCGGGGATAGCGCGCATAGAAATc                            <  2:392941/90‑1 (MQ=255)                                          aaCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATcc                           >  1:61578/1‑90 (MQ=255)                                          aaCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATcc                           <  2:144007/90‑1 (MQ=255)                                          aaCTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATcc                           <  2:201587/90‑1 (MQ=255)                                                tcgCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCAtt                     >  1:252813/1‑90 (MQ=255)                                                      cAAAGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAgggg               <  1:96985/90‑1 (MQ=255)                                                        aaGCATATCCTCGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGcc             <  2:395476/90‑1 (MQ=255)                                                                   cGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGc  >  2:116668/1‑90 (MQ=255)                                                                   cGATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGAGAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGc  >  2:115996/1‑90 (MQ=255)                                                                    gATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCg                          <  1:7620/65‑1 (MQ=255)                                                                    gATTTTCTCCTGCGACATTCCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCg                          >  2:7620/1‑65 (MQ=255)                                                                                       |                                                                      GCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGC  >  NZ_CP009273/353939‑354094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑