Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,085,073 G→C G194A (GGC→GCC)  rsmE → 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,085,0730GC80.0% 19.6 / 1.1 10G194A (GGC→GCC) rsmE16S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (3/5);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCC  >  NZ_CP009273/3084999‑3085138
                                                                          |                                                                 
gaaTCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGttt                                                    >  1:376687/1‑90 (MQ=255)
              gcgcCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGTTTATCGGCAGATGaaa                                      <  1:133704/90‑1 (MQ=255)
                   aGTAACAGCATCAATCCGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGcc                                 <  1:404000/90‑1 (MQ=255)
                                     ttGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCaa               >  1:87571/1‑90 (MQ=255)
                                          gTTACCGGTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTAc          <  1:218729/90‑1 (MQ=255)
                                               cGGTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGata     <  2:354325/90‑1 (MQ=255)
                                                 gTTGAACGCGTCCGCCTGCTGATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATc   >  1:37922/1‑90 (MQ=255)
                                                  ttGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATcc  <  2:402544/90‑1 (MQ=255)
                                                                     gATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATc   <  1:365911/70‑1 (MQ=255)
                                                                     gATTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATc   >  2:365911/1‑70 (MQ=255)
                                                                          |                                                                 
GAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCC  >  NZ_CP009273/3084999‑3085138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑