Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,275,191	T→G	intergenic (+207/‑144)	BW25113_RS16255 → / → agaS	amidohydrolase family protein/AgaS family sugar isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,275,191	0	T	G	100.0%	18.7 / NA	7	intergenic (+207/‑144)	BW25113_RS16255/agaS	amidohydrolase family protein/AgaS family sugar isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/4);  total (3/4)

CGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACC  >  NZ_CP009273/3275133‑3275261                                                           |                                                                       cgcATCCAGCAATCCCGTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAg                                         >  2:306978/1‑90 (MQ=255)        aGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGtttcttt                                  <  2:353591/90‑1 (MQ=255)                        tCCTTTATCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATc                  >  1:19320/1‑90 (MQ=255)                          cTTTATCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCaa                >  1:331765/1‑90 (MQ=255)                              aTCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGaa            <  2:162054/90‑1 (MQ=255)                              aTCTTTTCTTTCAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGaa            <  2:324252/90‑1 (MQ=255)                                        tcAACGATCACAAATTTCGGTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCAcc  <  1:75732/90‑1 (MQ=255)                                                           |                                                                       CGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACC  >  NZ_CP009273/3275133‑3275261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑