| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,597,860 | C→T | T36T (ACC→ACT) | rsmD → | 16S rRNA (guanine(966)‑N(2))‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,597,860 | 0 | C | T | 82.3% | 39.4 / 1.8 | 17 | T36T (ACC→ACT) | rsmD | 16S rRNA (guanine(966)‑N(2))‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (7/7); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
AATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCG > NZ_CP009273/3597785‑3597933 | aaTCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGa < 2:95126/90‑1 (MQ=255) aGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGt > 1:389659/1‑90 (MQ=255) cTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGAc < 1:213655/90‑1 (MQ=255) tCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACg > 1:129216/1‑90 (MQ=255) cccGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGc < 1:2910/90‑1 (MQ=255) cccGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGc > 2:59128/1‑90 (MQ=255) ggTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCa < 2:55618/90‑1 (MQ=255) ggTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCa < 2:57904/90‑1 (MQ=255) ggTTCCTGATAGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCa < 2:94335/90‑1 (MQ=255) tCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATg > 2:184965/1‑90 (MQ=255) aGCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGAt < 2:139563/90‑1 (MQ=255) gCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGAtt > 1:246255/1‑90 (MQ=255) gCCCAGGTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGAtt > 1:309746/1‑90 (MQ=255) gCCCAGGTCTGCGCCCCACCACTGACCGCGCACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGAtt > 1:229247/1‑90 (MQ=255) gTCTGCGCCCCACCACTGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCg > 2:119579/1‑90 (MQ=255) ccaccaCCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTc > 1:223884/1‑45 (MQ=255) ccaccaCCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTc < 2:223884/45‑1 (MQ=255) | AATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGTCTGGATTGCTTCG > NZ_CP009273/3597785‑3597933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |