Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	52.5 / NA	17	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/3);  total (14/3)

TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAAC  >  NZ_CP009273/3896539‑3896693                                                                                    |                                                                         tGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGa                                                                   >  1:211018/1‑90 (MQ=255)          cATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGAt                                                          >  1:164652/1‑90 (MQ=255)          cATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGAt                                                          <  1:167279/90‑1 (MQ=255)          cATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGAt                                                          >  1:131443/1‑90 (MQ=255)            ttAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGc                                                               >  2:348509/1‑83 (MQ=255)            ttAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGc                                                               <  1:348509/83‑1 (MQ=255)                                cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                    >  2:45720/1‑90 (MQ=255)                                cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                    >  2:385341/1‑90 (MQ=255)                                    ttATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCAcc                                >  1:69511/1‑90 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                         >  1:41830/1‑90 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                         >  2:402748/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg        >  2:2586/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg        >  2:302241/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg        >  1:259495/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg        >  1:175417/1‑90 (MQ=255)                                                                gggCATAATGC‑CGAAGTGGGTCGGGGCCCGCTCAATAACCCAAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa    <  1:402748/90‑1 (MQ=255)                                                                  gCGTAATGCTCGAAGT‑GGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAAc  >  1:25913/1‑90 (MQ=255)                                                                                    |                                                                         TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGT‑GATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAAC  >  NZ_CP009273/3896539‑3896693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑