| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,922,863 | A→G | V34A (GTC→GCC) | viaA ← | ATPase RavA stimulator ViaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,922,863 | 0 | A | G | 90.0% | 51.6 / ‑2.6 | 20 | V34A (GTC→GCC) | viaA | ATPase RavA stimulator ViaA |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (5/13); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGC > NZ_CP009273/3922779‑3922946 | ttCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGgccgcc < 2:118649/90‑1 (MQ=255) aGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGc < 1:238835/90‑1 (MQ=255) aGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGc < 1:240598/90‑1 (MQ=255) tGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTg < 2:128879/90‑1 (MQ=255) gCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCcagc < 2:83639/79‑1 (MQ=255) gCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCcagc > 1:83639/1‑79 (MQ=255) ggggAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGatca < 2:132236/90‑1 (MQ=255) gAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCAtct > 1:9114/1‑90 (MQ=255) atcatcAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTc < 1:51673/90‑1 (MQ=255) atcaGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGAt < 1:362193/90‑1 (MQ=255) atcaGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGAt > 2:278143/1‑90 (MQ=255) caGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCgatga > 1:119666/1‑75 (MQ=255) caGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCgatga < 2:119666/75‑1 (MQ=255) gATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATcc > 1:201397/1‑90 (MQ=255) cGTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGcc < 2:331631/90‑1 (MQ=255) gTGGGAATTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCa < 2:146368/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 1:148360/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 2:201397/90‑1 (MQ=255) aaTTTTTCAAAGAAGGCCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 2:327102/90‑1 (MQ=255) aaTTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATc < 1:125295/90‑1 (MQ=255) aagaagACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGc < 1:330057/90‑1 (MQ=255) | TTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCCAGCAGTGCGATGATCATCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGC > NZ_CP009273/3922779‑3922946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |