| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,004,326 | T→G | V256G (GTG→GGG) | yigL → | sugar/pyridoxal phosphate phosphatase YigL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,004,326 | 0 | T | G | 100.0% | 64.5 / NA | 20 | V256G (GTG→GGG) | yigL | sugar/pyridoxal phosphate phosphatase YigL |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (10/10); total (10/10) | |||||||||||
ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAA > NZ_CP009273/4004247‑4004404 | aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTAt < 1:40247/90‑1 (MQ=255) gTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAc > 1:326674/1‑90 (MQ=255) tGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAct < 1:73322/90‑1 (MQ=255) cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTAt > 2:123435/1‑90 (MQ=255) gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAActc < 1:204780/82‑1 (MQ=255) gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAActc > 2:204780/1‑82 (MQ=255) gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATc > 1:101007/1‑90 (MQ=255) cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt > 2:36100/1‑74 (MQ=255) cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt < 1:36100/74‑1 (MQ=255) gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGcc > 1:184605/1‑41 (MQ=255) gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGcc < 2:184605/41‑1 (MQ=255) gCTGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt < 2:292325/90‑1 (MQ=255) gAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTc < 2:379235/90‑1 (MQ=255) gATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCt > 1:327592/1‑90 (MQ=255) gTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATAc < 2:327592/90‑1 (MQ=255) aTGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCt > 2:235470/1‑90 (MQ=255) cgacgaCGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTc > 2:141509/1‑70 (MQ=255) cgacgaCGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTc < 1:141509/70‑1 (MQ=255) cgacgaCGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACaa < 1:361190/90‑1 (MQ=255) cgacgaCGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACaa > 2:324567/1‑90 (MQ=255) | ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAA > NZ_CP009273/4004247‑4004404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |