Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	448,331	G→T	A224D (GCC→GAC)	ampG ←	muropeptide MFS transporter AmpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	448,331	0	G	T	100.0%	38.4 / NA	12	A224D (GCC→GAC)	ampG	muropeptide MFS transporter AmpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/5);  total (7/5)

GTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTT  >  NZ_CP009273/448268‑448411                                                                |                                                                                 gTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGc                                                        <  2:97590/90‑1 (MQ=255)        tCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCag                                                      >  1:130614/1‑85 (MQ=255)        tCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCag                                                      <  2:130614/85‑1 (MQ=255)        tCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTg                                                 >  1:321408/1‑90 (MQ=255)          aTGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGca                                               >  1:27461/1‑90 (MQ=255)          aTGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGca                                               >  2:295709/1‑90 (MQ=255)                                      gCACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGca                   >  1:257504/1‑90 (MQ=255)                                       cACGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGcac                  >  2:366059/1‑90 (MQ=255)                                        aCGATAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGcaca                 <  2:361849/90‑1 (MQ=255)                                           aTAAGAAGCAAAATAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGa              >  2:81993/1‑90 (MQ=255)                                                       aTAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggtt  <  1:366059/90‑1 (MQ=255)                                                       aTAAGCCAGTCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggtt  <  2:13242/90‑1 (MQ=255)                                                                |                                                                                 GTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTT  >  NZ_CP009273/448268‑448411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑