| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,153,816 | T→C | S84P (TCA→CCA) | btuB → | TonB‑dependent vitamin B12 receptor BtuB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,153,816 | 0 | T | C | 100.0% | 40.0 / NA | 14 | S84P (TCA→CCA) | btuB | TonB‑dependent vitamin B12 receptor BtuB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/8); total (6/8) | |||||||||||
CCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG > NZ_CP009273/4153731‑4153901 | ccGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATc < 1:102774/90‑1 (MQ=255)ccGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATc < 1:64479/90‑1 (MQ=255) cGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTCAGGGCAGCTCCCATCt > 2:34717/1‑90 (MQ=255) aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc < 1:177769/90‑1 (MQ=255) aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc < 2:389739/90‑1 (MQ=255) ccTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgcg < 1:349646/90‑1 (MQ=255) ggTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTCAGGTCAGCTCCCCCCTCTTTTTTTTCGCgggaa > 2:141324/1‑87 (MQ=255) gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCGCGGCAGCCCCCCTCTCTTTTTTTTTGCGGGAGAAAAGCCAGt > 2:74746/1‑90 (MQ=255) gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGGACAAAAGCCAGTCAtgt > 2:84054/1‑90 (MQ=255) gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAAAGCCAGTCAtgt > 1:403092/1‑90 (MQ=255) cGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTg < 2:156168/90‑1 (MQ=255) ttCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTTAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGgtgt < 1:237645/90‑1 (MQ=255) gTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCgg < 1:236283/90‑1 (MQ=255) gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGCGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTgg > 2:267438/1‑90 (MQ=255) | CCGCTGGCAGTCGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG > NZ_CP009273/4153731‑4153901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |