Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,336,254	T→G	D297A (GAC→GCC)	fumB ←	class I fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,336,254	0	T	G	100.0%	39.8 / NA	13	D297A (GAC→GCC)	fumB	class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/6);  total (7/6)

CTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCA  >  NZ_CP009273/4336168‑4336329                                                                                       |                                                                            cTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGt                                                                          <  2:63010/90‑1 (MQ=255)          tACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGt                                                                 >  2:136054/1‑90 (MQ=255)                            agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTgc                                                <  1:240803/89‑1 (MQ=255)                            agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTgc                                                >  2:240803/1‑89 (MQ=255)                               cGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTgcgcgc                                            <  2:46675/90‑1 (MQ=255)                               cGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTgcgcgc                                            >  1:320448/1‑90 (MQ=255)                                    aTGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGa                                                                    >  1:280859/1‑61 (MQ=255)                                    aTGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGGCGTGCGCGa                                                                    <  2:280859/61‑1 (MQ=255)                                                            tGACGTGCCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCcagc               <  1:210293/90‑1 (MQ=255)                                                            tGACGTGCCAGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCcagc               >  2:210293/1‑90 (MQ=255)                                                                     aGACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGTGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGt      >  1:174135/1‑90 (MQ=255)                                                                      gACGGATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGtt     <  1:210602/90‑1 (MQ=255)                                                                         ggATAACGCGAATGGCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCa  >  1:229238/1‑90 (MQ=255)                                                                                       |                                                                            CTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCA  >  NZ_CP009273/4336168‑4336329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑