Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,421,635	C→G	A56P (GCA→CCA)	ytfE ←	iron‑sulfur cluster repair protein YtfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,421,635	0	C	G	100.0%	51.2 / NA	17	A56P (GCA→CCA)	ytfE	iron‑sulfur cluster repair protein YtfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (6/11);  total (6/11)

GCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAG  >  NZ_CP009273/4421555‑4421714                                                                                 |                                                                                gcccACGAGGATATGGTCGATGATTTCTGCCAGTGGGGCGCTACGCCAGTCTTTCACAATCGGTTGTTCAGCGAGCTTGGGCAGTTCAGc                                                                        <  2:358277/87‑1 (MQ=255)      cGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCaa                                                                   >  1:256999/1‑90 (MQ=255)        atgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg                                                                 <  2:365251/90‑1 (MQ=255)               ggTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCaa                                                          <  1:9226/90‑1 (MQ=255)                  cGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACAt                                                       >  1:402842/1‑90 (MQ=255)                  cGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACAt                                                       >  2:383871/1‑90 (MQ=255)                    atgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg                                                                 >  1:223414/1‑78 (MQ=255)                    atgatTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATg                                                                 <  2:223414/78‑1 (MQ=255)                          tCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCt                                               >  2:299669/1‑90 (MQ=255)                                     ggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTgccgc                                    >  1:35742/1‑90 (MQ=255)                                     ggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTgccgc                                    <  2:216211/90‑1 (MQ=255)                                           aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCcgcgcg                              <  2:143506/90‑1 (MQ=255)                                              ccAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCgcgcgcgc                           <  2:402842/90‑1 (MQ=255)                                                aGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCa                         <  2:156413/90‑1 (MQ=255)                                                                  gttCAGCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCcac       <  1:137034/90‑1 (MQ=255)                                                                       gCGAGCTTTGGCAGTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag  <  2:9265/90‑1 (MQ=255)                                                                       gCGAGCTTTGGCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCAcagcag  <  1:295428/90‑1 (MQ=255)                                                                                 |                                                                                GCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAG  >  NZ_CP009273/4421555‑4421714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑