Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 465,464 T→C S380P (TCG→CCG)  mdlA → SmdA family multidrug ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273465,4640TC100.0% 43.9 / NA 14S380P (TCG→CCG) mdlASmdA family multidrug ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

CGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGC  >  NZ_CP009273/465378‑465547
                                                                                      |                                                                                   
cgcTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGc                                                                                  <  2:121485/90‑1 (MQ=255)
    ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCAt                                                                              >  1:129779/1‑90 (MQ=255)
    ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCAt                                                                              >  2:277672/1‑90 (MQ=255)
                                           gggTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCtt                                       >  2:147683/1‑90 (MQ=255)
                                               aTCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCAt                                   <  2:394209/90‑1 (MQ=255)
                                                   gCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGata                               <  1:129646/90‑1 (MQ=255)
                                                     gggCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATAtt                             <  1:225671/90‑1 (MQ=255)
                                                     gggCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATAtt                             >  1:285514/1‑90 (MQ=255)
                                                                   cGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGtt               <  1:136808/90‑1 (MQ=255)
                                                                    ggCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAg                                                     >  1:362265/1‑51 (MQ=255)
                                                                    ggCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAg                                                     <  2:362265/51‑1 (MQ=255)
                                                                         aaGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACt         >  2:252902/1‑90 (MQ=255)
                                                                             aCCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGAt     >  2:324363/1‑90 (MQ=255)
                                                                                cTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc  >  1:219946/1‑90 (MQ=255)
                                                                                      |                                                                                   
CGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGC  >  NZ_CP009273/465378‑465547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: