Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,484,479	A→G	S14G (AGT→GGT)	idnK →	gluconokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,484,479	0	A	G	100.0%	58.2 / NA	18	S14G (AGT→GGT)	idnK	gluconokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/8);  total (10/8)

GGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAA  >  NZ_CP009273/4484398‑4484561                                                                                  |                                                                                   ggCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTaaa                                                                            <  1:366325/90‑1 (MQ=255)    gtagtaTCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGGGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGGAAAACa                                                                         >  2:158901/1‑90 (MQ=255)      agtaTCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACAtt                                                                       <  2:44094/90‑1 (MQ=255)            gcagcaGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTgg                                                                 >  1:18673/1‑90 (MQ=255)            gcagcaGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTgg                                                                 >  1:28183/1‑90 (MQ=255)            gcagcaGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTACTTTGATGGGCGTTTCAGGGGGTGGGAAAACATTAATTgg                                                                 >  2:84535/1‑90 (MQ=255)                cagGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGc                                                             <  1:351840/90‑1 (MQ=255)                            ttCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCcgcg                                                 >  1:207708/1‑90 (MQ=255)                                 gagaTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGttatt                                            >  2:240348/1‑90 (MQ=255)                                            tGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAAt                                 <  2:359250/90‑1 (MQ=255)                                              gCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCt                                     <  2:153625/84‑1 (MQ=255)                                              gCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCt                                     >  1:153625/1‑84 (MQ=255)                                                    gAAAGCTTTATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGAt                         <  2:265578/90‑1 (MQ=255)                                                           ttATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACg                  <  2:375269/90‑1 (MQ=255)                                                           ttATTTTGATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACg                  >  1:154914/1‑90 (MQ=255)                                                                  gATGGGCGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCa           >  1:13850/1‑90 (MQ=255)                                                                        cGTTTCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGc     >  1:372849/1‑90 (MQ=255)                                                                           ttCAGGGGGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCaa  <  2:28183/90‑1 (MQ=255)                                                                                  |                                                                                   GGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAA  >  NZ_CP009273/4484398‑4484561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑