| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 575,614 | A→G | intergenic (+72/+93) | BW25113_RS02885 → / ← ylcI | hypothetical protein/YlcI/YnfO family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 575,614 | 0 | A | G | 96.2% | 80.1 / ‑4.1 | 26 | intergenic (+72/+93) | BW25113_RS02885/ylcI | hypothetical protein/YlcI/YnfO family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (18/7); total (19/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC > NZ_CP009273/575528‑575699 | cTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAg > 2:98492/1‑90 (MQ=255) gATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCa > 1:63344/1‑90 (MQ=255) ccATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCa > 2:247384/1‑90 (MQ=255) tAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAAtt > 1:40820/1‑90 (MQ=255) aCTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTAc < 2:332718/90‑1 (MQ=255) gCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGc > 2:162519/1‑90 (MQ=255) aaTCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAAt > 1:79226/1‑90 (MQ=255) tCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGc > 2:270941/1‑90 (MQ=255) ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt > 1:156037/1‑90 (MQ=255) ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt > 1:133163/1‑90 (MQ=255) ttAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAg > 2:371180/1‑90 (MQ=255) tgACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTa > 2:104642/1‑90 (MQ=255) gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCaa > 1:279039/1‑61 (MQ=255) gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCaa < 2:279039/61‑1 (MQ=255) gagCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAata > 1:256431/1‑90 (MQ=255) gCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc < 2:27130/84‑1 (MQ=255) gCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc > 1:27130/1‑84 (MQ=255) gCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATc > 2:121124/1‑90 (MQ=255) acacGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTcc < 1:371180/90‑1 (MQ=255) cAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTc > 2:68536/1‑90 (MQ=255) aGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCg > 1:175684/1‑90 (MQ=255) gtcactgtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg > 2:241368/1‑62 (MQ=255) gtcactgtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg < 1:241368/62‑1 (MQ=255) tcactgtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGga < 2:214746/90‑1 (MQ=255) gtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAc > 1:234439/1‑90 (MQ=255) caGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAcgc < 2:180096/90‑1 (MQ=255) | CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC > NZ_CP009273/575528‑575699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |