| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 587,468 | A→C | D389E (GAT→GAG) | nrfB ← | cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 587,468 | 0 | A | C | 100.0% | 32.3 / NA | 11 | D389E (GAT→GAG) | nrfB | cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (3/8); total (3/8) | |||||||||||
GATGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAG > NZ_CP009273/587381‑587543 | gATGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTc < 2:224151/90‑1 (MQ=255) aaCCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACaa < 2:210727/90‑1 (MQ=255) ttAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagca < 2:98524/90‑1 (MQ=255) acaGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCcagcaacagca < 1:89394/90‑1 (MQ=255) cagGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAg < 2:133841/90‑1 (MQ=255) tCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGa < 2:268335/90‑1 (MQ=255) aTTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCTAAAGCTGGatc > 1:336311/1‑90 (MQ=255) aGAAAGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGAc < 1:208785/90‑1 (MQ=255) aGGAAATGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAg > 1:95157/1‑90 (MQ=255) aaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACaa < 1:129877/84‑1 (MQ=255) aaTGCCAGGCCTCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACaa > 2:129877/1‑84 (MQ=255) | GATGCGGTTAACCATCAAACCAAAGTTTAGCCACAGCAGGGTCATTAACCATGCGCTGCCGCTGAAAATAGAAAGGAAATGCCAGGCATCGGGCCACAAACTTTCATACGCCAGCAACAGCAAAAGCTGGATCATCACCAGCATCGCGAGGAAGCTGACAAAG > NZ_CP009273/587381‑587543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |