Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	701,647	G→A	P33P (CCG→CCA)	glnS →	glutamine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	701,647	0	G	A	100.0%	58.1 / NA	18	P33P (CCG→CCA)	glnS	glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/7);  total (11/7)

GATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCC  >  NZ_CP009273/701597‑701729                                                   |                                                                                   gATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGaaa                                             >  1:28809/1‑90 (MQ=255)    gAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAatct                                          >  2:358194/1‑90 (MQ=255)            ggCCAGTCGTAAGCACACCACAGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCt                                  >  1:22352/1‑90 (MQ=255)                 gTGGTAAGCACACCACAGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACt                             >  1:355131/1‑90 (MQ=255)                             ccacAGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGccc                 <  2:355131/90‑1 (MQ=255)                                caGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAgg              <  1:214005/90‑1 (MQ=255)                                caGTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAgg              <  1:395114/90‑1 (MQ=255)                                  gTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGa                                >  1:283198/1‑70 (MQ=255)                                  gTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGa                                <  2:283198/70‑1 (MQ=255)                                  gTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            >  2:89358/1‑90 (MQ=255)                                  gTACACACCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            <  2:22352/90‑1 (MQ=255)                                       cacCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            >  1:90836/1‑85 (MQ=255)                                       cacCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            <  2:90836/85‑1 (MQ=255)                                       cacCCGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATaa       >  2:102476/1‑90 (MQ=255)                                         cccGTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAg     >  1:364553/1‑90 (MQ=255)                                            gTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            >  2:187890/1‑80 (MQ=255)                                            gTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGAc            <  1:187890/80‑1 (MQ=255)                                            gTTTCCCACCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGcc  >  1:110437/1‑90 (MQ=255)                                                   |                                                                                   GATGAAGATCTGGCCAGTGGTAAGCACACCACAGTACACACCCGTTTCCCGCCGGAGCCGAATGGCTATCTGCATATTGGCCATGCGAAATCTATCTGCCTGAACTTCGGGATCGCCCAGGACTATAAAGGCC  >  NZ_CP009273/701597‑701729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑