Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 901,740 G→A A157V (GCG→GTG)  ybjS ← NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273901,7400GA90.0% 24.8 / ‑3.6 10A157V (GCG→GTG) ybjSNAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (6/3);  total (7/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

CCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCT  >  NZ_CP009273/901660‑901823
                                                                                |                                                                                   
ccAGACGGGGCATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAAGAGGAAAGCGCGTTTGCGGATTCACCTGCGaaa                                                                            >  2:361797/1‑90 (MQ=255)
          aaTAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGAt                                                                  >  1:51588/1‑90 (MQ=255)
           aTAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATc                                                                 <  1:319153/90‑1 (MQ=255)
                          tGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGaaa                                                                            <  1:185672/64‑1 (MQ=255)
                          tGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGaaa                                                                            >  2:185672/1‑64 (MQ=255)
                                        aCTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGc                                    >  2:122789/1‑90 (MQ=255)
                                                                gcgcGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg            >  1:83365/1‑90 (MQ=255)
                                                                gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg            >  1:286348/1‑90 (MQ=255)
                                                                gcgcGTTTGTGGATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAgg            >  2:378587/1‑90 (MQ=255)
                                                                          ggATTCACCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAAtct  <  2:264503/90‑1 (MQ=255)
                                                                                |                                                                                   
CCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCT  >  NZ_CP009273/901660‑901823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑