Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,062,210	T→C	G390G (GGT→GGC)	agp →	bifunctional glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,062,210	0	T	C	100.0%	40.1 / NA	13	G390G (GGT→GGC)	agp	bifunctional glucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)

GAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATA  >  NZ_CP009273/1062132‑1062247                                                                               |                                      gagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGt                                       >  1:154075/1‑79 (MQ=255) gagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGt                                       <  2:154075/79‑1 (MQ=255)  agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGAc                           >  2:387819/1‑90 (MQ=255)   gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                          >  2:296395/1‑90 (MQ=255)     gCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCt                        >  1:344123/1‑90 (MQ=255)             gTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGttt                <  2:388300/90‑1 (MQ=255)                       gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg      >  1:369751/1‑90 (MQ=255)                           aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  <  2:13334/90‑1 (MQ=255)                           aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  <  2:264987/90‑1 (MQ=255)                                 tAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  <  1:403498/84‑1 (MQ=255)                                 tAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  >  2:403498/1‑84 (MQ=255)                                        gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGc                         <  1:234838/54‑1 (MQ=255)                                        gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGc                         >  2:234838/1‑54 (MQ=255)                                                         gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  <  1:248125/60‑1 (MQ=255)                                                         gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa  >  2:248125/1‑60 (MQ=255)                                                                               |                                      GAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATA  >  NZ_CP009273/1062132‑1062247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑