| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 108,362 | A→G | F242S (TTC→TCC) | zapD ← | cell division protein ZapD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 108,362 | 0 | A | G | 88.9% | 17.6 / ‑2.9 | 9 | F242S (TTC→TCC) | zapD | cell division protein ZapD |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (6/2); total (7/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
ACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGG > NZ_CP009273/108284‑108440 | accaccGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGt < 2:144359/90‑1 (MQ=255) ttCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTAcc > 1:87481/1‑90 (MQ=255) aCCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGc < 1:145845/90‑1 (MQ=255) ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg > 2:269927/1‑90 (MQ=255) ttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACg > 2:92327/1‑90 (MQ=255) ttAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCtt > 1:307263/1‑90 (MQ=255) tAGCAACAGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa > 1:122216/1‑90 (MQ=255) aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCTGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg > 2:258344/1‑90 (MQ=255) aGGCCAGTTCGGAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg > 2:280084/1‑90 (MQ=255) | ACCACCGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGG > NZ_CP009273/108284‑108440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |