Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,402,000	A→G	I18T (ATC→ACC)	dgcM ←	diguanylate cyclase DgcM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,402,000	0	A	G	100.0%	36.8 / NA	12	I18T (ATC→ACC)	dgcM	diguanylate cyclase DgcM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

TAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTT  >  NZ_CP009273/1401926‑1402069                                                                           |                                                                      tAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTgg                                                        <  2:314984/90‑1 (MQ=255)                  aTCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTa                                       >  1:348206/1‑90 (MQ=255)                     aaCAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGt                                        <  1:12290/86‑1 (MQ=255)                     aaCAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGt                                        >  2:12290/1‑86 (MQ=255)                     aaCAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTg                                    >  1:110250/1‑90 (MQ=255)                         gTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGt                                >  1:224450/1‑90 (MQ=255)                                cgcgCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                         <  1:299848/90‑1 (MQ=255)                                                       gTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTAtttt  >  2:1966/1‑90 (MQ=255)                                                        ttCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                         >  1:114868/1‑66 (MQ=255)                                                        ttCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                         <  2:114868/66‑1 (MQ=255)                                                          ccTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTcc                                             <  1:245384/44‑1 (MQ=255)                                                          ccTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTcc                                             >  2:245384/1‑44 (MQ=255)                                                                           |                                                                      TAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTT  >  NZ_CP009273/1401926‑1402069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑