Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,434,377	A→G	F222S (TTC→TCC)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,434,377	0	A	G	88.9%	18.5 / ‑3.3	9	F222S (TTC→TCC)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (4/4);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTG  >  NZ_CP009273/1434318‑1434430                                                            |                                                      ccTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACcgc                         <  2:391078/90‑1 (MQ=255)   tGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCgg                       <  1:150381/90‑1 (MQ=255)    gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa                      >  1:372322/1‑90 (MQ=255)    gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa                      <  2:372322/90‑1 (MQ=255)              gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg            <  2:125027/90‑1 (MQ=255)                      ccGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGGTCCGGGt    >  2:73846/1‑90 (MQ=255)                        gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg  >  1:37222/1‑90 (MQ=255)                        gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg  >  2:205645/1‑90 (MQ=255)                        gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg  <  1:325371/90‑1 (MQ=255)                                                            |                                                      CCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTG  >  NZ_CP009273/1434318‑1434430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑