Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,524,815	T→C	intergenic (‑54/‑28)	BW25113_RS25600 ← / → BW25113_RS07655	protein YncO/ISAs1 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,524,815	0	T	C	100.0%	14.8 / NA	6	intergenic (‑54/‑28)	BW25113_RS25600/BW25113_RS07655	protein YncO/ISAs1 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/2);  total (4/2)

ATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACA  >  NZ_CP009273/1524739‑1524898                                                                             |                                                                                    aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATa                                                                        >  2:316607/1‑90 (MQ=255)                    tCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACTCCAGGTGAg                                                     <  1:60110/90‑1 (MQ=255)                                  tttGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                       >  2:161067/1‑90 (MQ=255)                                       tttAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                  <  1:211517/90‑1 (MQ=255)                                                    ggTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTAAGTGa                     >  2:128830/1‑90 (MQ=255)                                                                       ttGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaaca  >  1:240307/1‑90 (MQ=255)                                                                             |                                                                                    ATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACA  >  NZ_CP009273/1524739‑1524898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑