Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,576,388	A→G	S132P (TCC→CCC)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,576,388	0	A	G	85.7%	10.5 / ‑2.7	7	S132P (TCC→CCC)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (4/2);  total (4/3)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

CTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAT  >  NZ_CP009273/1576311‑1576436                                                                              |                                                 cTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGc                                      <  1:100776/90‑1 (MQ=255)        tCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAg                               >  1:216126/1‑90 (MQ=255)                     aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                  >  1:253702/1‑90 (MQ=255)                           cAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCgtgt                                        <  1:353855/62‑1 (MQ=255)                           cAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCgtgt                                        >  2:353855/1‑62 (MQ=255)                                    cgggAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCa   >  2:105732/1‑90 (MQ=255)                                     gggAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAt  <  2:253702/90‑1 (MQ=255)                                                                              |                                                 CTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAT  >  NZ_CP009273/1576311‑1576436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑