Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,615,188	A→C	S69R (AGT→AGG)	eamA ←	O‑acetylserine/cysteine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,615,188	0	A	C	100.0%	18.3 / NA	7	S69R (AGT→AGG)	eamA	O‑acetylserine/cysteine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/5);  total (2/5)

ACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTT  >  NZ_CP009273/1615103‑1615232                                                                                      |                                             aCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGa                                          <  2:200035/90‑1 (MQ=255)      tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt                                     <  1:133399/90‑1 (MQ=255)      tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt                                     <  1:154550/90‑1 (MQ=255)      tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt                                     <  1:399042/90‑1 (MQ=255)      tgtgcctgtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGtt                                     <  2:309023/90‑1 (MQ=255)                       cGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCcagcag                    >  2:379041/1‑90 (MQ=255)                                         cATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAACCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACttt  >  2:296362/1‑90 (MQ=255)                                                                                      |                                             ACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTT  >  NZ_CP009273/1615103‑1615232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑