Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,795,564	T→A	T422S (ACT→TCT)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,795,564	0	T	A	100.0%	17.3 / NA	6	T422S (ACT→TCT)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/5);  total (1/5)

AACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGACTAAA  >  NZ_CP009273/1795499‑1795622                                                                  |                                                           aaCCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAgaggag                                                        <  1:175758/70‑1 (MQ=255) aaCCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAgaggag                                                        >  2:175758/1‑70 (MQ=255)  aCCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCg                                   <  1:285489/90‑1 (MQ=255)            ccGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAg                         <  2:84279/90‑1 (MQ=255)                 tCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTg                    <  1:33939/90‑1 (MQ=255)                     cACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACa                <  1:204619/90‑1 (MQ=255)                               caTTTCGTCGCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGAc      <  2:195391/90‑1 (MQ=255)                                   tcgtcgCTGCCAATACGTTTTTCAGGACGAGAGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGACTaaa  >  2:7724/1‑90 (MQ=255)                                                                  |                                                           AACCGCCAGGTCCGCCTCAGCACGATCCCACATTTCGTCGCTGCCAATACGTTTTTCAGGACGAGTGGAGAGTTTGACGACGATCTTCTCGAAGCCAAAAGTGCTGTACATATCATAGACTAAA  >  NZ_CP009273/1795499‑1795622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑