Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,868,213	T→C	F128S (TTC→TCC)	yeaK →	mischarged aminoacyl‑tRNA deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,868,213	0	T	C	100.0%	11.3 / NA	5	F128S (TTC→TCC)	yeaK	mischarged aminoacyl‑tRNA deacylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/3);  total (2/3)

AGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATA  >  NZ_CP009273/1868139‑1868257                                                                           |                                             aGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTtgatga                               <  2:361259/90‑1 (MQ=255)      gtgtCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCg                          >  2:366728/1‑90 (MQ=255)                    aTCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGc            <  1:277359/90‑1 (MQ=255)                        ccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGc        <  2:152456/90‑1 (MQ=255)                              tCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATa  >  2:174151/1‑90 (MQ=255)                                                                           |                                             AGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATA  >  NZ_CP009273/1868139‑1868257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑