Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	264,689	(A)5→4	intergenic (‑18/+311)	ykfA ← / ← perR	GTPase family protein/LysR substrate‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	264,685	0	A	.	100.0%	113.5 / NA	27	intergenic (‑14/+315)	ykfA/perR	GTPase family protein/LysR substrate‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/18);  total (9/18)

TTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT  >  NZ_CP009273/264615‑264769                                                                       |                                                                                     ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                                  >  1:536803/1‑90 (MQ=255) ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                                                  >  1:990034/1‑90 (MQ=255)   gCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGaa                                                                >  2:720933/1‑90 (MQ=255)        aGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTg                                                           <  2:350353/90‑1 (MQ=255)                aaGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTccg                                                   <  1:162987/90‑1 (MQ=255)                   cGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTccgccg                                                >  2:687488/1‑90 (MQ=255)                   cGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTccgccg                                                >  2:29718/1‑90 (MQ=255)                     aTTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGtt                                              >  1:44766/1‑90 (MQ=255)                      ttGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTa                                             >  1:532809/1‑90 (MQ=255)                         cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt                                          <  2:48709/90‑1 (MQ=255)                             aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt                                      <  1:753415/90‑1 (MQ=255)                             aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt                                      <  2:398256/90‑1 (MQ=255)                              aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTa                                     <  2:956597/90‑1 (MQ=255)                              aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTa                                     <  2:440369/90‑1 (MQ=255)                              aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTa                                     <  2:34356/90‑1 (MQ=255)                                   ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                                <  2:725193/90‑1 (MQ=255)                                   ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc                                <  1:471986/90‑1 (MQ=255)                                       aaCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag                            <  1:720933/90‑1 (MQ=255)                                       aaCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag                            <  2:536803/90‑1 (MQ=255)                                         ccTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtg                          <  1:524378/90‑1 (MQ=255)                                         ccTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTAAGATTCCGCCGTTAATTGCGTATGTTCAGAGtg                          <  1:889082/90‑1 (MQ=255)                                           ttCAGAATTATTCATCCCTACCCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  2:763953/90‑1 (MQ=37)                                           ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  2:300381/90‑1 (MQ=255)                                           ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  2:44766/90‑1 (MQ=255)                                           ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat                        <  2:185366/90‑1 (MQ=255)                                            tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgatg                       >  1:171466/1‑90 (MQ=255)                                                                 cctcTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAtttttt  >  2:70147/1‑90 (MQ=255)                                                                       |                                                                                     TTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT  >  NZ_CP009273/264615‑264769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑