Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,103,8650A.97.4% 157.0 / ‑1.3 39intergenic (+65/+84)BW25113_RS15410/yghDtRNA‑Phe/GspM family type II secretion system protein YghD
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (14/24);  total (14/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTT  >  NZ_CP009273/3103778‑3103950
                                                                                       |                                                                                     
ttAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAGTAACCTTCACGaaa                                                                                     >  1:837812/1‑90 (MQ=255)
   gATTCCGAGTTCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGCCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaa                                                                                  >  2:966105/1‑90 (MQ=255)
    aTTCCGAGTCCGGTCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaat                                                                                 <  1:870892/90‑2 (MQ=255)
    aTTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaat                                                                                 <  1:400908/90‑2 (MQ=255)
    aTTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaat                                                                                 <  1:626359/90‑2 (MQ=255)
        cGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGc                                                                            <  1:665570/90‑1 (MQ=255)
             ccGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTtata                                                                       >  2:810942/1‑90 (MQ=255)
             ccGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTtata                                                                       >  1:493467/1‑90 (MQ=255)
              cGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      <  2:961142/90‑1 (MQ=255)
              cGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      <  1:886373/90‑1 (MQ=255)
              cGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      <  2:697038/90‑1 (MQ=255)
              cGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      <  1:966105/90‑1 (MQ=255)
               gggCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      <  1:919464/89‑1 (MQ=255)
               gggCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATaa                                                                      >  2:919464/1‑89 (MQ=255)
                      acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                              <  2:52234/90‑1 (MQ=255)
                      acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                              <  1:95175/90‑1 (MQ=255)
                      acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                              <  1:514534/90‑1 (MQ=255)
                              ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTAc                                                      >  1:971674/1‑90 (MQ=255)
                                    aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                >  1:278761/1‑90 (MQ=255)
                                      ccGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCa                                              <  2:993014/90‑1 (MQ=255)
                                       cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc                                             >  2:91577/1‑90 (MQ=255)
                                       cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc                                             >  2:80503/1‑90 (MQ=255)
                                         cccACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGt                                           <  1:230317/90‑1 (MQ=255)
                                              aaGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACg                                      <  2:991546/90‑1 (MQ=255)
                                                   gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                 >  1:144377/1‑90 (MQ=255)
                                                   gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                 <  1:602444/90‑1 (MQ=255)
                                                   gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                 <  2:278761/90‑1 (MQ=255)
                                                   gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                 >  2:271714/1‑90 (MQ=255)
                                                         tttcTTTTTGATCTGACAATAACCTTCACGAAAAAATTAGCTTTTAAAGTCTGGGGGGATTACTCTCGCCACGTTAAAGAGAGGAAtttt                           >  1:164096/4‑90 (MQ=255)
                                                            cTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTAtt                        <  1:171825/90‑1 (MQ=255)
                                                            cTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTAtt                        <  2:971674/90‑1 (MQ=255)
                                                            cTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTAtt                        <  1:17050/90‑1 (MQ=255)
                                                                     cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt               <  1:367821/90‑1 (MQ=255)
                                                                     cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt               <  1:810942/90‑1 (MQ=255)
                                                                     cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt               <  2:988658/90‑1 (MQ=255)
                                                                     cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt               <  2:936828/90‑1 (MQ=255)
                                                                             aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt       <  2:182414/90‑1 (MQ=255)
                                                                             aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt       <  1:627412/90‑1 (MQ=255)
                                                                             aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt       <  1:793344/90‑1 (MQ=255)
                                                                              aCCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt       <  2:418606/90‑1 (MQ=255)
                                                                                 ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACtt   >  2:809807/1‑90 (MQ=255)
                                                                                 ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACtt   >  1:92336/1‑90 (MQ=255)
                                                                                  tCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACttt  >  2:956859/1‑90 (MQ=255)
                                                                                  tCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACttt  >  2:863428/1‑90 (MQ=255)
                                                                                       |                                                                                     
TTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTT  >  NZ_CP009273/3103778‑3103950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: