| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 510,152 | Δ1 bp | coding (164/459 nt) | ybbJ ← | NfeD family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,151 | 0 | C | . | 91.7% | 37.2 / ‑2.5 | 12 | coding (165/459 nt) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (5/6); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACC > NZ_CP009273/510063‑510228 | tgtgTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGca < 2:318766/90‑2 (MQ=255) gcgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGc > 2:137738/1‑90 (MQ=255) gccgcGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCAccag < 1:705174/90‑1 (MQ=255) gACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGa < 2:196933/90‑1 (MQ=255) ccATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGaccacc < 1:821341/90‑1 (MQ=255) ccATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGaccacc < 1:997042/90‑1 (MQ=255) caccacAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTCCCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCgg < 1:999625/90‑1 (MQ=255) gCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCAc > 2:511331/1‑90 (MQ=255) gAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcg > 2:610111/1‑90 (MQ=255) gTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc > 1:597778/1‑90 (MQ=255) gTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc > 1:983869/1‑90 (MQ=255) ggCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACaaa < 2:73327/90‑1 (MQ=255) aCATTCCCCCTTGCC‑CCCCCAACCCAGCGGCCCCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAAcc < 1:987762/90‑1 (MQ=255) | TGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACC > NZ_CP009273/510063‑510228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |