Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,076,7230T.92.3% 88.5 / 3.3 26intergenic (+454/‑89)putP/BW25113_RS05295sodium/proline symporter PutP/FTR1 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (20/4);  total (21/5)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

TTTCTACCCTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGG  >  NZ_CP009273/1076635‑1076807
                                                                                        |                                                                                    
tttCTACCCTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGtt                                                                                     >  2:148627/1‑90 (MQ=255)
      cccTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGttttcttt                                                                               <  1:360198/90‑5 (MQ=255)
          ggAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCg                                                                          >  2:683236/1‑90 (MQ=255)
               aatCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATg                                                                     >  2:867561/1‑90 (MQ=255)
               aatCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATg                                                                     >  1:537402/1‑90 (MQ=255)
                     ttATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATc                                                               >  2:856172/1‑90 (MQ=255)
                          tCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTAt                                                          >  2:324738/1‑90 (MQ=255)
                            ccTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCa                                                        >  2:510415/1‑90 (MQ=255)
                              ttGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACt                                                      >  1:648465/1‑90 (MQ=255)
                              ttGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTATGTTTTCTTTGCCGTAATGATAATCGCTATCACt                                                      >  2:812707/1‑90 (MQ=255)
                               tGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTg                                                     >  1:197349/1‑90 (MQ=255)
                                 gCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCg                                                   >  1:259112/1‑90 (MQ=255)
                                  cATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGa                                                  <  2:537402/90‑1 (MQ=255)
                                     tACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGAttt                                               >  1:431107/1‑90 (MQ=255)
                                      aCCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTa                                              >  1:786780/1‑90 (MQ=255)
                                          ctctTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACttt                                          >  2:440004/1‑90 (MQ=255)
                                           tctTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACtttt                                         >  2:603634/1‑90 (MQ=255)
                                                gTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTg                                    >  2:65291/1‑90 (MQ=255)
                                                    aCATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATa                                >  1:187069/1‑90 (MQ=255)
                                                      aTTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACtt                                           >  1:846313/1‑77 (MQ=255)
                                                      aTTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACtt                                           <  2:846313/77‑1 (MQ=255)
                                                          cAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGa                          <  2:328296/90‑1 (MQ=255)
                                                          cAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGa                          >  1:231214/1‑90 (MQ=255)
                                                                 atTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGAtt                            >  2:639286/1‑82 (MQ=255)
                                                                 atTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGAtt                            <  1:639286/82‑1 (MQ=255)
                                                                            aTTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAg        >  2:468706/1‑90 (MQ=255)
                                                                              tCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAggg      >  2:107621/1‑90 (MQ=255)
                                                                                  cTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAgggtggg  <  2:197349/90‑1 (MQ=255)
                                                                                        |                                                                                    
TTTCTACCCTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGG  >  NZ_CP009273/1076635‑1076807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: