| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,240,670 | Δ1 bp | coding (55/441 nt) | BW25113_RS06225 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,240,667 | 0 | G | . | 93.3% | 53.6 / ‑1.1 | 15 | coding (52/441 nt) | BW25113_RS06225 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (9/5); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAA > NZ_CP009273/1240587‑1240745 | tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAg > 2:507505/1‑90 (MQ=255) aaTTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAgg < 1:657349/90‑1 (MQ=255) ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGtt > 1:105371/1‑90 (MQ=255) cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCt < 1:512070/90‑1 (MQ=255) gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTg > 2:960079/1‑90 (MQ=255) ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGGTGCGCtt > 2:537091/1‑90 (MQ=255) ttCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACAttt < 1:507505/90‑1 (MQ=255) aaataaatATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAAtt < 1:493778/90‑1 (MQ=255) aGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGc < 1:473894/90‑1 (MQ=255) cAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGGTTTTGGTGCGCTTTTTTAAAATTTTGAATTTATTAAACCTTTTTGCAGATTaa > 1:789975/1‑90 (MQ=255) aGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTAGAAATGATCAATCCTTCTGGCAGATTAAg > 1:326148/1‑90 (MQ=255) tCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTgcgc > 1:336819/1‑45 (MQ=255) tCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTgcgc < 2:336819/45‑1 (MQ=255) aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTTTTTAAAATTTAAAAATGATCAAACCTTTTGGCAGATTAAACCaa > 2:697034/1‑90 (MQ=255) ggCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAAAATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaaa > 1:110661/1‑90 (MQ=255) | TTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAA > NZ_CP009273/1240587‑1240745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |