| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,417,788 | (C)6→7 | coding (69/120 nt) | BW25113_RS26130 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,417,782 | 1 | . | C | 88.2% | 42.8 / 1.6 | 17 | coding (75/120 nt) | BW25113_RS26130 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (6/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA > NZ_CP009273/1417693‑1417862 | ttGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCGCCGCCTCCGGCGTGGATAATGCAG‑‑CCt < 2:640992/90‑1 (MQ=18) cTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCT‑cccccc > 2:903442/1‑90 (MQ=33) cgGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCT‑CCCCCCGACTGGCAGac < 1:210061/90‑1 (MQ=33) ggTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGac < 2:123074/90‑1 (MQ=255) cAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAAc < 1:371900/90‑1 (MQ=255) tcagtcagTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAAACACCGCTGAACGGGATTATTTCa > 2:737395/1‑90 (MQ=11) cagtGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTc > 2:427390/1‑90 (MQ=255) gCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCggg > 1:403749/1‑90 (MQ=255) gTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGaa < 1:352265/90‑1 (MQ=14) gTGAAGCCACCGCCGCCTCCGGCGTTGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGaa < 1:831554/90‑1 (MQ=14) tGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAAc < 2:65650/90‑1 (MQ=255) gAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACg > 1:529143/1‑90 (MQ=255) ccaccaCCGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTg < 2:529143/90‑1 (MQ=255) accGCCTCCGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAat < 1:427390/90‑1 (MQ=255) cGGCGTGGATAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAgg > 2:675947/1‑90 (MQ=255) gTGGATAATGCAGCCCCC‑CCCCCCGTCTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc < 2:693476/90‑1 (MQ=21) tAATGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTg > 2:691861/1‑90 (MQ=255) aTGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa < 1:499605/90‑1 (MQ=255) aTGCAG‑‑CCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGaa < 2:972251/90‑1 (MQ=255) | TTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAG‑‑CCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCCAACTTGAA > NZ_CP009273/1417693‑1417862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |