| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,555,008 | 0 | T | G | 52.9% | ‑1.2 / 11.7 | 19 | D60A (GAC→GCC) | ddpB | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base G (9/0); total (17/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCA > NZ_CP009273/1554923‑1555095 | cGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAg > 2:592284/1‑90 (MQ=255) gACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTc > 1:499778/1‑90 (MQ=255) gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGGTGCTggg > 1:128336/1‑89 (MQ=255) aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCCGTCCCCGTTGCTGGGGAatat > 1:389541/1‑90 (MQ=255) cACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGCCCCAGTTGGTGGGGAATATTTTCCACCATAg > 1:511523/1‑90 (MQ=255) ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGGGAATATTTTCCACTATACCa > 2:733143/1‑90 (MQ=255) ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTTCTGGCGGATATTTTTCACGATAGCa > 1:100442/1‑90 (MQ=255) ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCTGGCGAAAATTTTTCCCGATAGCa > 1:17224/1‑90 (MQ=255) ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGAGGCTGGGCCCGGCCCACTTGCTGGCGCAAATTTTCCGCCATAGcc > 1:126130/1‑89 (MQ=255) ggTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcgc > 2:166804/1‑90 (MQ=255) gCTGACGTAGGGTTAAAACTGTACATACAGTGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTc < 1:225778/90‑1 (MQ=255) gCTGACGTAGCGGTAAAACTGTACATACAGTGGCT‑GTCCAGTCCCAGTTGCTGGGGAATATTTTCCCCGATAGCATCGCTGGCGCGGGCa > 2:261188/1‑90 (MQ=255) gTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCTGGGGAAAATTTTCCCCGATAGCATCGCTGGCGCGGGCACCCGc > 1:39824/1‑90 (MQ=255) cGGTAAAACTGTACATACAGTGGCTGGGTCCGTCCCCGGTGCTGGCGAAAATTTTTCACCAAAACATCCCCTGCGCGGGcccccccccat > 1:656846/1‑87 (MQ=255) acatacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa > 2:324784/1‑90 (MQ=255) acatacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAAAATTTTTCACGATAACATCGCTGGCGCGGGCACCCCCCAGTAACCGCGCCGGa > 1:422360/1‑90 (MQ=255) gTGGCTGGCCCAGCCCCGGTTGCGGGCAAATTTTTCCCACGATAGCATCGCGGGCGGGGTCCCCCGCCAGTAACCGCGCCGGATCACCAg < 1:166804/90‑1 (MQ=255) tGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACCATAGCATCGCTGGCGCGGGCACCCGCCCGGAACCGCGCCCGGTCACCCgg > 1:143210/1‑90 (MQ=255) ggTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCa > 2:345695/1‑90 (MQ=255) | CGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCA > NZ_CP009273/1554923‑1555095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |