| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,029,116 | 0 | A | C | 56.2% | 9.0 / 17.5 | 16 | G175G (GGT→GGG) | prfB | peptide chain release factor 2 |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGC > NZ_CP009273/3029027‑3029200 | cTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATa > 1:389229/1‑90 (MQ=255)cTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATa > 1:819892/1‑90 (MQ=255) ccAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACtt > 2:320919/1‑90 (MQ=255) aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCa > 2:866501/1‑90 (MQ=255) cGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa > 1:463671/1‑90 (MQ=255) gCGTACCCGCGGGATTTTTTGACCGTCACGTT‑TTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 1:265714/90‑1 (MQ=255) gCGTAATCGCGGGATTTTTTGTTCGTCACGTT‑TTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 2:255289/90‑1 (MQ=255) gCGTAACCGCGGGATTTTTTGTTCTTCATGTT‑TTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 1:658451/90‑1 (MQ=255) gCGAAATCGCGGGATTTTTTGCTCTTCACTTA‑TTT‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 1:146929/90‑1 (MQ=255) tAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGAt > 2:416811/1‑90 (MQ=255) cGGAGTTTTTGATCGTCA‑GGA‑TTTAAACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc < 1:822482/90‑1 (MQ=255) cGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc > 2:400456/1‑90 (MQ=255) tgataTTTTGCTCCTC‑CGGATTTT‑ACTCCCCGCCCCTTCCCCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCa < 1:929172/86‑1 (MQ=255) tttttGACCGTAAGGAT‑TTT‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc < 1:366685/89‑1 (MQ=255) tGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat > 1:573099/1‑90 (MQ=255) cGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacat > 1:598655/1‑90 (MQ=255) gTCACGAT‑TTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata < 2:877847/90‑1 (MQ=255) taaaTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGc < 2:243186/88‑1 (MQ=255) | CTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGA‑TTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGC > NZ_CP009273/3029027‑3029200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |