| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 64.3% | ‑1.0 / 27.0 | 28 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (1/9); new base T (18/0); total (19/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC > NZ_CP009273/3175879‑3176046 | cTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGttt > 2:265153/1‑90 (MQ=255) ggTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCgg < 1:55987/90‑1 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGGGCGGTTTTCCGCGt > 1:806037/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGGGCGGTTTTTGGCGTTTT‑C‑G‑GGGAAAAAcc > 1:964029/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGCGCCCCGTTTTTTTGCGCGTTTTTTCGCGTTTT‑CGG‑GGCAAAAAc > 1:279333/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGGTTTTCCGCCTTCTCC‑C‑GGCAAAAAc > 1:387057/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGGTTCTCGGCGTTCT‑C‑G‑GGCaaaaaac > 2:99542/1‑88 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGGCGCGTTTTTTTTGGCGGTTTTTTGCGTTTT‑T‑GCGGCAAAAAccc > 1:760720/1‑90 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAAc < 1:354447/90‑1 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTCGGCGTTTT‑C‑G‑GGCAAAAAACCCCCAAcc > 2:758221/1‑90 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCT‑C‑G‑GGGAAAAAACCCCGAAcc > 2:354447/1‑90 (MQ=255) gTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCg < 2:880928/90‑1 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGGTTTTTCGCGTTTT‑C‑C‑GGGCAAAACACCCCAACCGCCTa > 1:707977/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGGTTTTCGGCGTTCT‑C‑G‑GGGAAAAAACCCCCAACCCCTTTCttgg > 1:173497/1‑87 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTTGCGCGTTTTTCTGCGTTCT‑C‑C‑GGCAAAAACCCCCCGACCCCTTTCTGGc > 1:64106/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCCCTTACTGGc > 2:387335/1‑90 (MQ=255) ctcATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTTCGCGGTTT‑C‑G‑GGCCAAAAACCCCGAACCGCTTTCTGGCg > 1:617371/1‑90 (MQ=255) ctcATTCTGACCATACTGGCGGGGGCAGCCCAGTTTTTTAGCGCGCTTTTCGGCGTTTT‑C‑G‑GGCCAAAACCCCCGAACCTCCTACTTGCg > 2:675314/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGGTTTTCGGCGGTCT‑C‑C‑GGGAAAAAACCTCGCACCCCTTACTGGCGtt > 1:625486/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGtt < 2:821743/90‑1 (MQ=255) ttCTGACCATACTGGCGGGGGGAGCCCCCCTTTTTTGGGCGTTTTTCCGCGGTCT‑C‑C‑GGCAAAAAACCCCCCACCGCCTTCTGGCGtttt > 1:772554/1‑90 (MQ=255) gACCATACTGGCGGGGGCAACCCCGGTTTTTGGCGCGTTTCTCGGCCTTCT‑C‑G‑GGCAAAAAACCCCCAACCCCCTACTGGCGttttttcc > 2:779632/1‑86 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:957824/90‑1 (MQ=255) gggggCAGCCACGGTTTTTTGGGCGTTTTTCGGCGTTCT‑C‑G‑GGGAAAAAACCTCGAACCGCTTTCTGGGGGTTTTGCTTGGGTTTgcggc > 1:24648/1‑90 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:476985/90‑1 (MQ=255) cccGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAACCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCAt < 1:565187/88‑1 (MQ=255) cGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGt < 2:398739/90‑1 (MQ=255) ttATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGc < 1:673860/90‑1 (MQ=255) aTTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctc < 1:63255/90‑1 (MQ=255) | CTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑C‑G‑GGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC > NZ_CP009273/3175879‑3176046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |