| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,726,648 | 0 | T | C | 55.0% | 7.4 / 20.0 | 20 | L363P (CTC→CCC) | xylG | D‑xylose ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base C (11/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCA > NZ_CP009273/3726562‑3726732 | cATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTA‑GGGAAAAAAATTATCCCcgc > 1:698930/1‑90 (MQ=255) cGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTcgccgc < 1:873707/90‑1 (MQ=255) gCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCa < 2:995430/90‑1 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAAAATTACCCTCGCCGCACTCAATAAAt > 2:348242/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGT‑GGTAAAAAAAATAACCCCGCCGCCCCCAATAAAt > 1:357369/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGT‑GGGGAAAAAAATACCCCCCCCCCCCCCAATaaaa > 1:901984/1‑89 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAAAATTACCCCCCCCCCCCTCAATAAATTTACCgg > 2:657513/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGT‑GGGAAAAAAATTAACCCCGCCCCCCTCAAAAAAATTACCgg > 1:346492/1‑90 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 2:815196/90‑1 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 1:445203/90‑1 (MQ=255) ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAAAAATACCCCCCCCCCCCCCAAAAAAATTACCGGTGGCa > 1:304693/1‑90 (MQ=255) aCAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGcc < 1:775816/90‑1 (MQ=255) aCAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAAAATTACCCCCCCCCCCCCCAAAAAAATTTCCCGGGGGGTTAGcc > 1:210499/1‑90 (MQ=255) aGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGT‑GGTAAAAAAATTACCCCCCCCCCCCTCAAAAAAATTTTCGGGGGGATTAGCCAg > 1:112724/1‑90 (MQ=255) gAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAAATAACCCCCCCCCCCCCAAAAAAATTAACCGGGGGC‑TTAGCCAGc > 1:193386/1‑90 (MQ=255) gAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGT‑GGGAAAAAAATTACCCCCCCCCCCCCCCATAAAATTTACGGGGGGATTAGCCAGc > 1:576323/1‑90 (MQ=255) cGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCaaa < 2:30425/90‑1 (MQ=255) tGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGGGGGATTAGCCAGCTTGATGACGCGGGAGAGCAAAAAttga > 2:162508/1‑87 (MQ=255) gTT‑GGTAAAAAAATTACCCCCCCCGCACTCAATAAATTTACCGGGGGGATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAAGTATTTTg > 1:325850/1‑90 (MQ=255) tACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCa > 1:139459/1‑90 (MQ=255) | CATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTT‑GGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCA > NZ_CP009273/3726562‑3726732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |