Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,840	T→A	V96E (GTG→GAG)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,840	0	T	A	100.0%	114.8 / NA	31	V96E (GTG→GAG)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/15);  total (16/15)

GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTT  >  NZ_CP009273/232753‑232923                                                                                        |                                                                                    gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   >  2:619680/1‑90 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  2:403660/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  2:316623/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  1:104109/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  1:501902/90‑1 (MQ=255) gTATTGCCGATAAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  2:692573/90‑1 (MQ=255)   aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATc                                                                                 <  1:452520/90‑1 (MQ=255)    ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATcc                                                                                >  1:778685/1‑90 (MQ=255)         gATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAAc                                                                           >  2:860450/1‑90 (MQ=255)             aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAg                                                                       <  1:354980/90‑1 (MQ=255)             aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAg                                                                       <  2:21528/90‑1 (MQ=255)                 tttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGtt                                                                   <  1:860663/90‑1 (MQ=255)                        gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATc                                                            <  1:890046/90‑1 (MQ=255)                        gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATc                                                            <  1:25542/90‑1 (MQ=255)                                cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTAt                                                    <  2:119834/90‑1 (MQ=255)                                   gTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGa                                                 >  2:498805/1‑90 (MQ=255)                                          gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGtt                                                                   >  1:44662/1‑65 (MQ=255)                                          gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGtt                                                                   <  2:44662/65‑1 (MQ=255)                                                 ccGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGt                                   <  1:443933/90‑1 (MQ=255)                                                    aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGc                                >  1:671776/1‑90 (MQ=255)                                                    aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGc                                >  1:614257/1‑90 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCt                               <  1:172641/77‑1 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCt                               >  2:172641/1‑77 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  2:330870/1‑90 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  2:464907/1‑90 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  2:170291/1‑90 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  1:532505/1‑90 (MQ=255)                                                                      ttCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa              <  2:335673/90‑1 (MQ=255)                                                                      ttCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa              <  2:614257/90‑1 (MQ=255)                                                                         gcggcgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGa           >  1:420027/1‑90 (MQ=255)                                                                             cgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTc                                     <  2:143642/60‑1 (MQ=255)                                                                             cgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTc                                     >  1:143642/1‑60 (MQ=255)                                                                             cgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa       >  2:376626/1‑90 (MQ=255)                                                                             cgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa       >  1:364835/1‑90 (MQ=255)                                                                             cgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa       >  2:584879/1‑90 (MQ=255)                                                                                  aGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt  <  1:521151/90‑1 (MQ=255)                                                                                  aGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt  <  1:860450/90‑1 (MQ=255)                                                                                        |                                                                                    GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTT  >  NZ_CP009273/232753‑232923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑