Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,131,004	A→G	intergenic (‑280/‑379)	wza ← / → yegH	polysaccharide export protein Wza/TerC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,131,004	0	A	G	100.0%	51.6 / NA	16	intergenic (‑280/‑379)	wza/yegH	polysaccharide export protein Wza/TerC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

TTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACG  >  NZ_CP009273/2130922‑2131087                                                                                   |                                                                                    ttttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAAc                                                                              <  2:185899/90‑1 (MQ=255)                 acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                              <  2:255875/90‑1 (MQ=255)                         aGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATg                                                      <  1:381756/90‑1 (MQ=255)                              aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAt                                                 >  2:889984/1‑90 (MQ=255)                               aTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAtt                                                <  2:786982/90‑1 (MQ=255)                                         cccTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGc                                      <  2:822424/90‑1 (MQ=255)                                                  cATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaata                             <  1:270497/90‑1 (MQ=255)                                                     gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTc                                                                           <  1:581794/41‑1 (MQ=255)                                                     gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTc                                                                           >  2:581794/1‑41 (MQ=255)                                                     gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                          >  2:473962/1‑90 (MQ=255)                                                     gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                          >  2:776777/1‑90 (MQ=255)                                                           gTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATgg                                                     <  1:759420/57‑1 (MQ=255)                                                           gTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATgg                                                     >  2:759420/1‑57 (MQ=255)                                                           gTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCaggag                    <  2:974/90‑1 (MQ=255)                                                                tgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAgt               >  2:925514/1‑90 (MQ=255)                                                                             ttaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACg  >  2:550406/1‑90 (MQ=255)                                                                                   |                                                                                    TTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACG  >  NZ_CP009273/2130922‑2131087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑