Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,220,044	A→G	S236P (TCG→CCG)	yohF ←	SDR family oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,220,044	0	A	G	100.0%	121.0 / NA	37	S236P (TCG→CCG)	yohF	SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/11);  total (26/11)

CAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGCAAG  >  NZ_CP009273/2219960‑2220129                                                                                     |                                                                                      cAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGcc                                                                                  >  2:493954/1‑90 (MQ=255)  aaTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGccc                                                                                 <  1:842949/90‑1 (MQ=255)   aTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCg                                                                                >  2:31838/1‑90 (MQ=255)   aTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCg                                                                                >  1:843625/1‑90 (MQ=255)   aTGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCg                                                                                >  2:533919/1‑90 (MQ=255)    tGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCgg                                                                               <  2:651985/90‑1 (MQ=255)           tCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTa                                                                        <  1:748240/90‑1 (MQ=255)                  aCCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTgcg                                                                 <  1:97377/90‑1 (MQ=255)                      cACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCt                                                             >  1:844635/1‑90 (MQ=255)                            gcTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAAc                                                       <  1:19123/90‑1 (MQ=255)                             cTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACa                                                      >  1:761136/1‑90 (MQ=255)                               aTTCTGGGTTGATCTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTgcg                                                                 <  1:579371/77‑1 (MQ=255)                               aTTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTgcg                                                                 >  2:579371/1‑77 (MQ=255)                               aTTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaaa                                                    <  1:88581/90‑1 (MQ=255)                                    gggTTGAACTGTGGATTCGCCACCATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAAc                                                       <  2:740338/82‑1 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAAc                                                       >  1:740338/1‑82 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                               >  1:747182/1‑90 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                               >  1:852168/1‑90 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                               >  1:892707/1‑90 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                               <  2:612510/90‑1 (MQ=255)                                    gggTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                               >  2:241329/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGTCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  2:575953/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  2:85686/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  2:335762/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  1:468201/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  1:27556/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  1:225949/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  1:164297/1‑90 (MQ=255)                                         gAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGGAATTTGCGCCCTCCGAACAAAGCCAcaccac                                          >  2:482461/1‑90 (MQ=255)                                              gtgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGc                                     >  1:780560/1‑90 (MQ=255)                                              gtgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGc                                     >  1:742439/1‑90 (MQ=255)                                              gtgGATTCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGc                                     >  2:850351/1‑90 (MQ=255)                                                    tCGCCAACATAAAGCCGCCATCCACTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCaa                               <  2:468201/90‑1 (MQ=255)                                                                            cTATCAACGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAAcgcc       >  2:796790/1‑90 (MQ=255)                                                                                 aaCGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCaa                               <  2:117561/61‑1 (MQ=255)                                                                                 aaCGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCaa                               >  1:117561/1‑61 (MQ=255)                                                                                 aaCGGCTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGCAAg  >  1:292141/1‑90 (MQ=255)                                                                                     |                                                                                      CAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGCCGCAAG  >  NZ_CP009273/2219960‑2220129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑