| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 259,276 | A→G | G35G (GGT→GGC) | ykfI ← | type IV toxin‑antitoxin system toxin YkfI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 259,276 | 0 | A | G | 94.7% | 115.6 / ‑2.4 | 38 | G35G (GGT→GGC) | ykfI | type IV toxin‑antitoxin system toxin YkfI |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (16/20); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGT > NZ_CP009273/259191‑259359 | tCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCAt < 2:365657/90‑1 (MQ=255) ggCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTg < 2:907304/90‑1 (MQ=255) ggCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTg < 1:792679/90‑1 (MQ=255) gCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGc < 1:814051/90‑1 (MQ=255) ggTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCcagcag < 2:135557/90‑1 (MQ=255) tGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGAc < 2:319089/90‑1 (MQ=255) gATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACg > 2:274666/1‑90 (MQ=255) gATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACg < 1:4783/90‑1 (MQ=255) tACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACgtg < 1:328998/90‑1 (MQ=255) ggCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATTGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAg > 1:916172/1‑90 (MQ=255) cGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACa > 1:20196/1‑90 (MQ=255) tgtTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGc > 2:556499/1‑90 (MQ=255) tgtTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGc > 2:540001/1‑90 (MQ=255) cTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGAc > 1:911015/1‑90 (MQ=255) cacaGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCAc < 2:972442/90‑1 (MQ=255) cacaGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCAc < 2:453298/90‑1 (MQ=255) caGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACgg > 1:25815/1‑90 (MQ=255) caGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAATGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCCCgg > 1:499500/1‑90 (MQ=21) caGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACgg > 1:785692/1‑90 (MQ=255) aGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGAc > 1:869702/1‑59 (MQ=255) aGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGAc < 2:869702/59‑1 (MQ=255) tGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGAcaggc < 1:180572/90‑1 (MQ=255) tGCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGAcaggc < 1:84759/90‑1 (MQ=255) gCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACg > 1:898331/1‑79 (MQ=255) gCAGAATGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACg < 2:898331/79‑1 (MQ=255) aGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGAcaggcagg < 2:785692/90‑1 (MQ=255) aTGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCt > 1:291105/1‑90 (MQ=255) tGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACAtt > 2:656154/1‑58 (MQ=255) tGGCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACAtt < 1:656154/58‑1 (MQ=255) gCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCa > 2:128085/1‑90 (MQ=255) gCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCa > 1:908165/1‑90 (MQ=255) gCGTGTCGTTTATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCa > 1:744780/1‑90 (MQ=255) tCGTTTATTGTCAGGCCATAGAGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCAccgcc > 2:541335/1‑90 (MQ=255) tttATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCAccgcccgc < 1:359210/90‑1 (MQ=255) tttATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCAccgcccgc < 2:17259/90‑1 (MQ=255) ttATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCt < 2:932925/90‑1 (MQ=255) ttATTGTCAGGCCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCt < 2:952596/90‑1 (MQ=255) tGTCAGGCCATAGAGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGt < 1:541335/90‑1 (MQ=255) | TCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGT > NZ_CP009273/259191‑259359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |